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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
16/12/2013 |
Data da última atualização: |
10/12/2014 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PEREIRA, G. S.; NUNES, E. S.; LAPERUTA, L. D. C.; BRAGA, M. F.; PENHA, H. A.; DINIZ, A. L.; MUNHOZ, C. F.; GAZAFFI, R.; GARCIA, A. A. F.; VIEIRA, M. L. C. |
Afiliação: |
ESALQ/USP; ESALQ/USP; ESALQ/USP; MARCELO FIDELES BRAGA, CPAC; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP. |
Título: |
Molecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Annals of Applied Biology, v. 162, n. 3, p. 347-361, 2013. |
ISSN: |
0003-4746 |
DOI: |
10.1111/aab.12028 |
Idioma: |
Inglês |
Conteúdo: |
One of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0 cM, with an average distance between adjacent markers of 3.8 cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping. MenosOne of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of w... Mostrar Tudo |
Thesagro: |
Maracujá; Marcador genético; Marcador molecular; Melhoramento genético vegetal; Polimorfismo Genético. |
Thesaurus Nal: |
Genetic improvement; Genetic markers; Genetic polymorphism; Passiflora alata; Passion fruits. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02903naa a2200373 a 4500 001 1973873 005 2014-12-10 008 2013 bl uuuu u00u1 u #d 022 $a0003-4746 024 7 $a10.1111/aab.12028$2DOI 100 1 $aPEREIRA, G. S. 245 $aMolecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species. 260 $c2013 520 $aOne of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0 cM, with an average distance between adjacent markers of 3.8 cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping. 650 $aGenetic improvement 650 $aGenetic markers 650 $aGenetic polymorphism 650 $aPassiflora alata 650 $aPassion fruits 650 $aMaracujá 650 $aMarcador genético 650 $aMarcador molecular 650 $aMelhoramento genético vegetal 650 $aPolimorfismo Genético 700 1 $aNUNES, E. S. 700 1 $aLAPERUTA, L. D. C. 700 1 $aBRAGA, M. F. 700 1 $aPENHA, H. A. 700 1 $aDINIZ, A. L. 700 1 $aMUNHOZ, C. F. 700 1 $aGAZAFFI, R. 700 1 $aGARCIA, A. A. F. 700 1 $aVIEIRA, M. L. C. 773 $tAnnals of Applied Biology$gv. 162, n. 3, p. 347-361, 2013.
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Embrapa Cerrados (CPAC) |
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Registro Completo
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
22/11/2002 |
Data da última atualização: |
22/11/2002 |
Autoria: |
CARVALHO JUNIOR, W. de; AMARAL, F. C. S. do; PEREIRA, N. R.; CHAGAS, C. da S.; FERNANDES Y GARCIA, M. E. |
Título: |
Potencialidade pedoclimática para a cultura do trigo (Triticum aestivum) no Estado do Rio Grande do Sul. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
In: REUNIAO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 13., 2000, Ilhéus. 500 anos de uso do solo no Brasil: [anais...]. Ilhéus: CEPLAC: CEPEC, 2000. |
Idioma: |
Português |
Notas: |
1 CD-ROM. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00654naa a2200169 a 4500 001 1337058 005 2002-11-22 008 2000 bl uuuu u00u1 u #d 100 1 $aCARVALHO JUNIOR, W. de 245 $aPotencialidade pedoclimática para a cultura do trigo (Triticum aestivum) no Estado do Rio Grande do Sul. 260 $c2000 500 $a1 CD-ROM. 700 1 $aAMARAL, F. C. S. do 700 1 $aPEREIRA, N. R. 700 1 $aCHAGAS, C. da S. 700 1 $aFERNANDES Y GARCIA, M. E. 773 $tIn: REUNIAO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 13., 2000, Ilhéus. 500 anos de uso do solo no Brasil: [anais...]. Ilhéus: CEPLAC: CEPEC, 2000.
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